how does one separate the living material in soil samples, including viruses, from the inert, non-living soil particles? Subsequently, once you have separated this microbiological fraction, how do you test for the presence of and/or differentiate among viruses, bacteria, protists, fungi, or parasitic worms? How are viruses, bacteria, protists, fungi, or parasitic worms different?
How do you separate living material in soil samples including viruses from the inert nonliving soil particles?computer repair
The larger organisms, such as nematodes and parasitic worms, can be sorted out in small quantites visually (under a microscope).
Bacteria are often removed by culturing them under various conditions, and then isolating the cultures until a pure culture is obtained.
Viruses are smaller than most soil particles and are not typically grown in vitro, so separating them requires more equipment and finesse.
All of these can be separated by gradient based ultracentrifugation, but this requires specailized equipment, skill, and chemicals.
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